In Native American society, lacrosse served several different purposes. The sport was used for conflict resolution, the training of young warriors, and as a religious ritual. Games could be played on a pitch over a mile wide and sometimes lasted for days. Early lacrosse balls were made out of deerskin, clay, stone, and sometimes wood. Lacrosse has played a significant role in the community and religious life of tribes across the continent for many years. Early lacrosse was characterized by deep spiritual involvement, befitting the spirit of combat in which it was undertaken. Those who took part did so in the role of warriors, with the goal of bringing glory and honor to themselves and their tribes. The game was said to be played "for the pleasure of the Creator."

Lacrosse has witnessed significant modifications since its origins in the 14th century, but many aspects of the sport remain the same. In the traditional Native American version, each team consisted of about 100 to 1,000 men on a field that stretched from about 500 yards to a couple of miles long. These lacrosse games lasted from sunup to sundown for two to three days straight. These games were played to settle inter-tribal disputes, to toughen young warriors in preparation for future combat and to give thanks to the Creator. The modern Ojibway verb 'to play Lacrosse' is baaga'adowe.

The game became known to Westerners when a French Jesuit Missionary, Jean de Brébeuf, saw the Iroquois tribesmen play it in 1636. He was the first to write about lacrosse and thus gave it its name. Some say the name originated from the French term for field hockey, le jeu de la crosse. Others suggest that it was named after the crosier, a staff carried by bishops.

In 1856, Dr. William George Beers, a Canadian dentist, founded Montreal Lacrosse Club and in 1867 he codified the game, shortening the length of each game and reducing the number of players to twelve per team. The first game played under Beers' rules was at Upper Canada College in 1867, with Upper Canada College losing to the Toronto Cricket Club by a score of 3–1. By the 1900s, high schools, colleges, and universities began playing the game. Lacrosse was contested as a demonstration sport in the 1928 and 1932 Olympics. On both occasions, rather than holding tryouts and sending an All-star amalgamation, the U.S. chose to be represented by the Johns Hopkins University Blue Jays.

In the United States, lacrosse had primarily been a regional sport centered in and around New England, upstate New York, Long Island and the Mid-Atlantic States. In recent years however, its popularity has started to spread south to Georgia and Florida, as well as west to Colorado, California, Texas, and the Midwest, spurred by the sport's increasing visibility in the media, the growth of college, high school, and youth (or "pee wee") programs throughout the country. The NCAA Men's Lacrosse Championship is the most attended NCAA Championship, outdrawing the Final Four of men's basketball. The growth of lacrosse was also facilitated by the introduction of plastic heads in the 1970s by Baltimore-based STX. This innovation reduced the weight and cost of the lacrosse stick, and allowed for faster passes and game play than traditional wooden sticks.

Up until the 1930s all lacrosse was played on large fields outdoors. Around this time the owners of Canadian hockey arenas invented a reduced version of the game, called box lacrosse, as a means to make more profit from their arena investments. Through this commercialization, in a relatively short period of time, box lacrosse became the dominant form of the sport in Canada. More recently field lacrosse has witnessed a revival in Canada as the Canadian University Field Lacrosse Association (CUFLA) began operating a collegiate men's league in 1985 that now includes 12 varsity teams. Lacrosse was officially declared Canada's National Summer Sport with the passage of the National Sports Act on May 12, 1994.

In 1987 a professional box lacrosse league was started called the Eagle Pro Box Lacrosse League. Eventually this league would change its name to the National Lacrosse League and grow to encompass lacrosse clubs in twelve cities scattered throughout the United States and Canada. In the summer of 2001 a professional field lacrosse league known as Major League Lacrosse (MLL) was inaugurated. Initially starting with six teams the MLL has grown to a total of ten clubs located in major metropolitan areas throughout the United States. On July 4th 2008 Major League Lacrosse set the professional lacrosse attendance record when 20,116 fans attended a game at Invesco Field in Denver, Colorado.